Thermo Scientific™ Pierce™ Iodination Reagent (1,3,4,6-tetrachloro-3α,6α-diphenyl-glycoluril)

Highlights:

• Extremely stable and virtually insoluble in water
• Solubility in chloroform permits plating aliquots of the reagent on the walls of the glass or plastic iodination vessels; plated vessels can be prepared in advance and stored in a dessicator
• Allows rapid iodination of proteins in an aqueous reaction mixture containing radioactive Na125I
• Side reactions are negligible
• No reducing reagent is required because reactions are terminated by simply decanting the reaction solution away from the plated iodination reagent
• Specific activities of 10,000cpm of 125I per microgram of protein and labeling efficiencies of 60% are easily achieved
• Can be used to iodinate phenolic groups on crosslinkers or other protein modification reagents either before or after a protein, peptide or cell surface is reacted with the modification reagent
• Small amounts of protein can be iodinated
• More efficient than Chloramine-T or Lactoperoxidase
• Iodinate phenolic groups on crosslinking reagents