Learn More
Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible
Shop All Thermo Scientific ProductsDescription
Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media. The beads have high binding capacity (up to 80 mg of protein per mL of settled beads) and can tolerate the high EDTA and reducing agent concentrations that are used to inhibit metalloproteases and stabilize sensitive intracellular proteins.
Features of the high-capacity EDTA-compatible Ni-IMAC MagBeads include:
- Chelator stable—stable in buffer containing 20 mM EDTA and DTT
- Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
- Exceptional high protein binding capacity—up to 80 mg/mL of settled beads
- High-performance—non-aggregating, superparamagnetic microparticles provide exceptional performance with manual and automated HTS applications (e.g., Thermo Scientific KingFisher instruments)
Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added benefits of having magnetic properties and being EDTA compatible. The MagBeads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications.
Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also compatible with simple benchtop purification applications using an appropriate magnetic stand. Automated instruments, however, are especially useful for higher throughput purification and screening of purification conditions.
Specifications
Specifications
| Bed Volume | 0.25 mL |
| Binding Capacity | Up to 80 mg of 6 xHis-tagged protein / mL of settled magnetic beads |
| Capacity (Metric) | 1 mL |
| Binding Property | Chelator Stable |
| Concentration | Slurry: 25% |
| Matrix | Magnetic Agarose |
| pH Range | 2 to 14 pH stability |
| Content And Storage | 1 mL of 25% suspension. Store at 2–8°C. |
| Shipping Condition | Wet Ice |
| Particle Size | 30 μm |
| Show More |
Frequently Asked Questions (FAQs)
The KingFisher product line has proven to give high-throughput, high purity, and consistency across samples when performing automated purifications using Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible.
Recovered protein can be run on SDS-PAGE and stained or analyzed by western blotting. Since the elution buffer contains 300 mM NaCl and 500 mM imidazole, recovered protein may need to be desalted or buffer-exchanged before proceeding to activity assays, mass spectrometry, or structural analyses.
The recommended storage buffer for the magnetic beads is 20% ethanol, 10 mM sodium acetate, pH 6.50. Please refer to the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024861_PierceHighCapNiIMACBeads_UG.pdf) for details.
Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible are alkali-stable. The beads can be exposed to NaOH, washed with water, neutralized with PBS, washed with water, and put back into storage buffer. Please refer to the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024861_PierceHighCapNiIMACBeads_UG.pdf) for details.
Up to 5 uses are possible with Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible.
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.