missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Magnetic Affinity Separation Particles
  6. Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible

Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible

Product Code. 17113269 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1 mL
5 mL
25 mL
100 mL
Unit Size:
0.25mL
1.25mL
25mL
Each
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
17113269 1 mL 0.25mL
17134759 1 mL 0.25mL
17123269 5 mL 1.25mL
17133269 25 mL Each
17143269 100 mL 25mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
5 options
This item is not returnable. View return policy
Product Code. 17113269 Supplier Thermo Scientific™ Supplier No. A50588

Please to purchase this item. Need a web account? Register with us today!

89.06 EUR valid until 2026-02-28
BEST PRICE on Promo! Use promo code "28001" to get your promotional price.
This item is not returnable. View return policy

Description

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media.

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media. The beads have high binding capacity (up to 80 mg of protein per mL of settled beads) and can tolerate the high EDTA and reducing agent concentrations that are used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC MagBeads include:

  • Chelator stable—stable in buffer containing 20 mM EDTA and DTT
  • Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
  • Exceptional high protein binding capacity—up to 80 mg/mL of settled beads
  • High-performance—non-aggregating, superparamagnetic microparticles provide exceptional performance with manual and automated HTS applications (e.g., Thermo Scientific KingFisher instruments)

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added benefits of having magnetic properties and being EDTA compatible. The MagBeads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications.

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also compatible with simple benchtop purification applications using an appropriate magnetic stand. Automated instruments, however, are especially useful for higher throughput purification and screening of purification conditions.

TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 0.25 mL
Binding Capacity Up to 80 mg of 6 xHis-tagged protein / mL of settled magnetic beads
Capacity (Metric) 1 mL
Binding Property Chelator Stable
Concentration Slurry: 25%
Matrix Magnetic Agarose
pH Range 2 to 14 pH stability
Content And Storage 1 mL of 25% suspension. Store at 2–8°C.
Shipping Condition Wet Ice
Particle Size 30 μm
Form Liquid Suspension
Product Line Pierce
Quantity 1 mL
Type Magnetic Affinity Bead
Column Type Bottle
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.