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Thermo Scientific™ Fluorescein-5-Maleimide

Product Code. 10078847 Shop All Thermo Scientific Products
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25 mg
Unit Size:
25mg
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10078847 25 mg 25mg
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Product Code. 10078847 Supplier Thermo Scientific™ Supplier No. 62245
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Description

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
TRUSTED_SUSTAINABILITY

Specifications

Quantity 25 mg
Product Type Maleimide
Solubility DMF (Dimethylformamide)
Content And Storage Store desiccated at -20°C, protect from moisture
Chemical Reactivity Thiol
Reactive Moiety Maleimide
Label or Dye FITC (Fluorescein)
Label Type Classic Dyes
Product Line Pierce
Emission 518
Excitation 494
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Safety and Handling

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Product Identifier
  • Fluorescein-5-Maleimide, 25 mg
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
 Hazard Statement
  • H302-Harmful if swallowed.
 Precautionary Statement
  • P270-Do not eat, drink or smoke when using this product.
  • P264-Wash thoroughly after handling.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : Fluorescein-5-maleimide

For Research Use Only. Not for use in diagnostic procedures.

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