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  6. Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Product Code. 11844071 Shop All Thermo Scientific Products
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Quantity:
10 mg
10 x 1 mg
5 g
25 g
Unit Size:
10mg
25g
5g
Pack of 10
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Product Code. 11844071

Brand: Thermo Scientific™ 77149

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Description

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

EDC is the abbreviation for 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, a crosslinker that has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5Æphosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS, Part No. 24510), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

Highlights:

  • Zero-length crosslinker
  • Numerous chemical conjugates can be synthesized via available –COOH and –NH2 groups
  • Amide bond formed provides a neutral linkage, which is ideal for preparing peptides and antigens
  • Adding Sulfo-NHS (Part No. 24510) enhances the coupling reaction at physiological pH conditions
  • Reactive groups: carbodiimide
  • Reactive toward: carboxyl and amino groups
TRUSTED_SUSTAINABILITY

Specifications

Chemical Reactivity Amine-Carboxyl
Cleavable No
Description EDC
Molecular Weight (g/mol) 191.7
PEGylated No
Spacer Arm Length 0.0 Å
Content And Storage Upon receipt store desiccated at -20°C.
Cell Permeability No
Shipping Condition Wet Ice
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Carbodiimide
Spacer Short (<10 Å)
Form Powder
Quantity 10 mg
Solubility Water
Format Standard, Single-use, Premium-grade
Water Soluble Yes
Product Type Crosslinker
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Safety and Handling

compliance-icons
Product Identifier
  • EDC 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochlor
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P271-Use only outdoors or in a well-ventilated area.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 1,3-Propanediamine,N-(ethylcarbonimidoyl)-N,N-di methyl-, monohydrochloride

For Research Use Only. Not for use in diagnostic procedures.

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