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Thermo Scientific™ Pierce™ Agarose ChIP Kit
Tienda Thermo Scientific Productos
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Quantity:
30 Reaction Kit
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Each
Descripción
Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.
This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.
Highlights:
- Simple and streamlined ChIP assay protocol (< 8 hours)
- Highly efficient isolation and lysis of nuclei
- Easy and reproducible enzymatic digestion
- Low-background and high-capacity Protein A/G agarose resin
- Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
- Fast and reproducible spin-column format; columns and collection tubes included
- High-recovery DNA purification
- Choose a complete ChIP assay kit or the separate chromatin preparation module
Includes:
Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls
Especificaciones
Especificaciones
| Binding Capacity | >50 mg Human IgG/mL resin binding capacity |
| Capacity (Metric) | ≥50 mg Human IgG per mL Resin Binding |
| Description | Pierce Agarose ChIP Kit |
| Content And Storage | Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C) • IP Dilution/Wash Buffer (5X), 11 mL (store at 4°C) • IP Wash Buffer 3 (5X), 4.5 mL (store at 4°C) • IP Elution Buffer (2X), 4.5 mL (store at 4°C) • Column Accessory Pack, 3 packs (store at room temperature or 4°C) • Microcentrifuge Tubes (1.5mL), 75 tubes (store at room temperature or 4°C) • DNA Clean-Up Column, 40 columns (store at room temperature or 4°C) • DNA Column Binding Solution, 30 mL (store at room temperature or 4°C) • DNA Column Wash Solution, 6 mL (store at room temperature or 4°C) • pH Indicator, 0.8 mL (store at room temperature or 4°C) • DNA Column Elution Solution, 5 mL (store at room temperature or 4°C) • Anti-RNA Polymerase II Antibody, 25 μL (store at -20°C) • Normal Rabbit IgG (1 mg/mL), 10 μL (store at -20°C) • ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL (store at -20°C) • Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C). |
| Product Line | Pierce |
| Quantity | 30 Reaction Kit |
| Type | ChIP Kit |
| Sufficient For | 30 Reactions |
| Format | Kit |
Seguridad y manipulación
Nombre del producto
- Pierce Agarose ChIP Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H336-May cause drowsiness or dizziness.
- H412-Harmful to aquatic life with long lasting effects.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P264-Wash hands thoroughly after handling.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Isopropyl alcohol, Guanidine hydrochloride, Sodium fluoride,Lithium chloride, Sodium chloride
For Research Use Only. Not for use in diagnostic procedures.
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