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Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Product Code. 10639209 Shop All Thermo Scientific Products
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Quantity:
200 reactions
Unit Size:
200 reactions
This item is not returnable. View return policy

Product Code. 10639209

Brand: Thermo Scientific™ F150BID

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230.10 EUR valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy

Description

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.

Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even formalin-fixed, paraffin-embedded (FFPE) human tissue samples. The Phusion Human Specimen Direct PCR Kit includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase, and PCR products obtained are perfectly suitable even for cloning purposes.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Two simple protocols for various applications
  • Extremely short PCR protocol times
  • Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

  • The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • Phusion Human Specimen DNA Polymerase, 80 μL
• 2X Phusion Human Specimen PCR Buffer, 2 x 1.25 mL
• Universal Control Primer Mix (25 μM), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL

Store at -20°C.
GC-Rich PCR Performance High
PCR Method Direct PCR
Polymerase Phusion Human Specimen DNA Polymerase
Reaction Speed Fast
Product Type Direct PCR Kit
Quantity 200 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR
Hot Start Built-In Hot Start
No. of Reactions 200 reactions
Overhang Blunt
Reaction Format Separate Components
Sample Type Human
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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