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  7. Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Artikelnummer. 16254551 Shop alle Thermo Scientific produkter
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Color:
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Green
No. of Reactions:
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2000 Reactions
500 Reactions
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100 units
2000 units
500 units
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Artikelnummer. Color No. of Reactions Pakningsstørrelse
16254551 Colorless 2000 Reactions 2000 units
10024537 Colorless 100 Reactions 100 units
10287787 Colorless 500 Reactions 500 units
13575160 Green 100 Reactions 100 units
13585160 Green 500 Reactions 500 units
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Artikelnummer. 16254551 Leverandør Thermo Scientific™ Leverandørnr. F530XL
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Beskrivelse

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

TRUSTED_SUSTAINABILITY

Tekniske data

Concentration 2 U/μL
Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion High Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Quantity 2000 units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR, High-fidelity PCR
Fidelity (vs. Taq) 52X
Hot Start No
No. of Reactions 2000 Reactions
Overhang Blunt
Reaction Format Standalone
Size (Final Product) 20 kb or less
Color Colorless
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For Research Use Only. Not for use in diagnostic procedures.

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