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Invitrogen™ Phospho-CREB (Ser133) Monoclonal Antibody (M415)

Product Code. 17896708
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Quantity:
100 μL
Unit Size:
100µL
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17896708 100 μL 100µL
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Product Code. 17896708 Supplier Invitrogen™ Supplier No. MA547622

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Description

Mouse Monoclonal Antibody

The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, as well as A431 treated with EGF and PC12 treated with NGF or Forskolin. This reactivity is not observed after lambda phosphatase treatment.

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer's Disease, histiocytoma and soft tissue melanoma.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Phospho-CREB (Ser133)
Applications Western Blot, Immunocytochemistry
Classification Monoclonal
Clone M415
Concentration ~0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene CREB1
Gene Accession No. P15337, P16220, Q01147
Gene Alias 2310001E10Rik; 3526402H21Rik; active transcription factor CREB; AV083133; cAMP response element binding protein 1; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; Creb-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein
Gene Symbols CREB1, CrebB
Host Species Mouse
Immunogen Clone M415 was generated from phospho-CREB (Ser-133) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-133 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site is also conserved in CREM (Ser-136) and ATF1 (Ser-63).
Purification Method Protein A
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 12912, 1385, 81646
Target Species Human, Mouse, Rat
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG1
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