missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. PCR Specific Buffers
  7. Thermo Scientific™ Phire Reaction Buffers

Thermo Scientific™ Phire Reaction Buffers

Product Code. 13515170 Shop All Thermo Scientific Products
Change view
Click to view available options
Chemical Name or Material:
Phire Green Reaction Buffer
Phire Reaction Buffer
Phire Reaction Buffer, detergent-free
Unit Size:
Pack of 4
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Chemical Name or Material unitSize
13515170 Phire Green Reaction Buffer Pack of 4
10165847 Phire Reaction Buffer Pack of 4
10525709 Phire Reaction Buffer, detergent-free Pack of 4
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
This item is not returnable. View return policy
Product Code. 13515170 Supplier Thermo Scientific™ Supplier No. F527L

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Includes

Phire Green Reaction Buffer

  • 5x Phire Green Reaction Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2 solution
  • DMSO

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel.

Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase. This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm.

The concentration of Mg2+ in Phire Reaction Buffer is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

Notes

  • For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.
TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Phire Green Reaction Buffer
Description Green
Concentration 5X
For Use With (Application) Hot-start PCR
Quantity 4 x 1.5 mL
Includes • 5X Phire Green Reaction Buffer, 4 x 1.5 mL
• 50 mM MgCl2, 2 x 1.5 mL
• DMSO, 0.5 mL

Store at -20°C.

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.