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Invitrogen™ Phalloidin Labeling Probes

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.

379.00 € - 962.00 €

Especificaciones

Product Type Phalloidin
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Productos 23
Código de producto Marca Color Excitation Wavelength Range Dye Type Precio Cantidad y disponibilidad  
Código de producto Marca Color Excitation Wavelength Range Dye Type Precio Cantidad y disponibilidad  
10174712
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Invitrogen™
A22281
Blue 346/442 nm Alexa Fluor™ 350
958.00 €
Each
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15932941
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Invitrogen™
A30104
Violet 405/450 nm Alexa Fluor™ Plus 405
542.00 €
Each
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10125092
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Invitrogen™
A12379
Green 495/518 nm Alexa Fluor™ 488
912.00 €
300 units
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10730355
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Invitrogen™
O7466
Green 496/520 nm Oregon Green™ 488
890.00 €
Each
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10615963
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Invitrogen™
F432
Green 496/516 nm FITC (Fluorescein)
886.00 €
Each
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10184712
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Invitrogen™
A22282
Yellow 531/554 nm Alexa Fluor™ 532
960.00 €
Each
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10063052
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Invitrogen™
R415
Red-orange 540/565 nm TRITC (Tetramethylrhodamine Isothiocyanate)
816.00 €
300 units
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10634053
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Invitrogen™
A22283
Orange 556/570 nm Alexa Fluor™ 546
910.00 €
Each
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10389132
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Invitrogen™
A34055
Orange 555/565 nm Alexa Fluor™ 555
890.00 €
Each
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16628105
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Invitrogen™
A30106
Orange 555/565 nm Alexa Fluor Plus 555
568.00 €
50µg
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10643853
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Invitrogen™
B3475
Red 558/569 nm BODIPY™
866.00 €
Each
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10135092
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Invitrogen™
A12380
Orange-red 578/600 nm Alexa Fluor™ 568
906.00 €
Each
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10751144
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Invitrogen™
A12381
Red 581/609 nm Alexa Fluor™ 594
908.00 €
Each
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10164852
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Invitrogen™
T7471
Red 591/608 nm Texas Red™
822.00 €
Each
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10053252
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Invitrogen™
A22284
Far-red 632/647 nm Alexa Fluor™ 633
906.00 €
5g
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10124892
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Invitrogen™
A34054
Far-red 633/647 nm Alexa Fluor™ 635
962.00 €
Each
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10656353
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Invitrogen™
A22287
Far-red 650/668 nm Alexa Fluor™ 647
934.00 €
5g
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16400815
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Invitrogen™
A30107
Far-red 650/668 nm Alexa Fluor Plus 647
568.00 €
Pack of 300
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10402322
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Invitrogen™
A22285
Near-infrared 663/690 nm Alexa Fluor™ 660
880.00 €
Each
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10656163
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Invitrogen™
A22286
Near-infrared 679/702 nm Alexa Fluor™ 680
914.00 €
Each
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16316512
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Invitrogen™
A30105
Near-infrared 758/784 nm Alexa Fluor™ Plus 750
542.00 €
Each
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10125002
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Invitrogen™
B7474
None None Biotin-XX
854.00 €
Each
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10718853
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Invitrogen™
P3457
None None Phalloidin (unlabeled)
379.00 €
1mg
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Descripción

Descripción

Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

  • High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
  • Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
  • Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
  • Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
  • Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
  • Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
  • Ease of use—staining is straightforward and quick
  • Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
  • Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

Información de órdenes

Shipping Condition: Room Temperature

Especificaciones

Especificaciones

Phalloidin
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Documentos

Documentos

Certificados

For Research Use Only. Not for use in diagnostic procedures.

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