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Description
Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Features of the PageRuler Unstained Protein Ladder include:
- Reference bands—50 kDa band is stronger than the others for easy orientation
- Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence
Applications
- Accurate protein sizing on SDS-polyacrylamide gels and western blots
Specifications
Specifications
| Content And Storage | Contents: eight vials of 250 μL each, proteins at 0.02 to 0.05 mg/mL of each Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol Storage: Upon receipt store at -20°C |
| Number of Markers | 14 |
| Ready to Load | Yes |
| Size Range | 10 to 200 kDa |
| Molecular Weight (g/mol) | 200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa |
| Quantity | 8 x 250 μL |
| Product Line | PageRuler |
| Product Type | Protein Ladder |
| Stain Type | Unstained |
| System Type | SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.
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