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OPTIZYME™ DpnI, Fisher BioReagents™
Brand: Fisher Bioreagents BP8009-1
Additional Details : Weight : 0.01000kg
Description
5'...G m6A^T C...3'
3'...C T^m6A G...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 100%
- Buffer 2: 100%
- Buffer 3: 50 - 100%
- Buffer 4: 100%
- Buffer 5: 50 - 100%
- 10mM Tris-HCl (pH 7.4 at 25°C), 400mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
- DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA.
- DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly.
- DpnI, Bsp143I and MboI all recognize the same sequence but have different methylation sensitivities and cleavage sites.
Methylation Effects:
Dam: Does not cut dam- DNA
- Gm6ATC
- 5'...Gm6A Tm5C G...3'
Specifications
| 10 U/μL | |
| 37°C | |
| >70% of pBR322 DNA fragments can be ligated and >95% of these can be re-cut after 50-fold over-digestion with DpnI | |
| Keep container tightly closed | |
| GA.TC | |
| Liquid |
| 10X OPTIZYME™ Buffer 4 | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4 at 25°C), 400mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| 500 U | |
| DpnI |