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OPTIZYME™ DNA Polymerase I, Fisher BioReagents™

54.91 € - 298.00 €

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Specifications

Concentration 10 U/μL
Color Colorless
pH 7.5
For Use With (Application) DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases
Storage Buffer OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM
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Description

Description

OPTIZYME™ DNA Pol I is a template-dependent DNA Polymerase that catalyzes 5'->3' DNA synthesis, and exhibits 3'->5'-exonuclease, 5'->3' exonuclease and RNase H activity.

Applications and Features:

  • OPTIZYME DNA Polymerase I labels DNA by nick translation in conjunction with DNase I.
  • Second-strand cDNA synthesis in conjunction with RNase H.
  • Incorporation of modified nucleotides (e.g., biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides).
  • Active in buffers for OPTIZYME restriction enzymes and reverse transcriptases.

Supplied with:
10X OPTIZYME DNA Pol I Buffer: 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl2, 10mM DTT

Source: E. Coli cells with a cloned polA gene for E.Coli

Quality Control:
Endodeoxyribonuclease-free

Specifications

Specifications

10 U/μL
7.5
OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM
E. coli cells with a cloned polA gene
Liquid
Colorless
DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases
-20°C
DNA Polymerase I
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