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Thermo Scientific™ Nunc™ MicroWell™ 96-Well, Nunclon Delta-Treated, Flat-Bottom, Optical Coverglass Base Microplate

Product Code. 10472222
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Product Code. 10472222 Supplier Thermo Scientific™ Supplier No. 164588
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Description

Color:Black, Clear Bottom; Sterility:Sterile; Lid:With Lid; Type: Optical Coverglass Base

Achieve optimal fluorescence and luminescence in cell culture and microscopic applications with the Thermo Scientific™ Nunc™ MicroWell™ 96-Well Optical-Bottom Plates with Coverglass Base, featuring a polystyrene black or white upper structure with a polystyrene film at the bottom of the plate.

Features:

  • Background, autoflorescence and crosstalk are improved
  • Black microplates for florescence measurements, with minimum back-scattered light and background fluorescence
  • White microplates for luminescence measurements, with maximum reflection and minimal autoluminescence
  • Cell culture surface is ideal for cell-based assays
  • CC2 surface modification closely mimics Poly-D-Lysine and is superior for attachment and growth of fastidious cells
  • Customized bar coding available
TRUSTED_SUSTAINABILITY

Specifications

Certifications/Compliance All microplates manufactured by Nunc meet the recommendation of American National Standards (ANSI) (ANSI/SBS 1-2004)
Lid With Lid
Array 8 x 12
Packaging 6 Pk., 30 Cases
Product Line Nunc
Color Black, Clear Bottom
Product Type Microplate
Material Polystyrene
No. of Wells 96
Sterility Sterile
Surface Treatment Cell Culture
Well Shape Flat Bottom (F-well)
No. per Case 30
No. per Pack 6
Quantity Case of 30
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Frequently Asked Questions (FAQs)

What is the difference between the Nunclon Delta surface and a tissue culture (TC) surface from a competitor?

Our Nunclon Delta surface for adherent cells is a standard tissue culture (TC) surface modification that makes the polystyrene surface more hydrophilic. This facilitates maximum adhesion for a broad range of cell types.

The Nunclon Delta surface is Thermo Scientific Nunclon Delta-certified, which is a Thermo Fisher proprietary certification that has a quality standard to help ensure consistency. Every lot of Thermo Scientific Nunclon Delta-certified cell cultureware is tested with Gibco media for:
- Less than 10% growth variation in L929, Hel299 and primary chick embryo cells
- Less than 15% variation colony formation of V79-4 cells

More information and all applicable products can be found in the "Product selection guide for every step of your cell culture workflow" brochure .
How does cell culturing on Nunc 384-Well Microplates that have a cell culture-treated surface compare with 96-Well Microplates that have a cell culture-treated surface?

The cell culture-treated surfaces are very similar but the wells of the Nunc 384-Well Microplates are smaller than the wells of the Nunc 96-Well Microplate.
Culture recommendations for the Nunc 384-Well Microplates are:
- 15 - 50 µL media per well
- 0.5 - 2.0 x 10^4 cells per well
Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on “top reader” instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom.

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