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Macherey-Nagel™ NucleoZOL, one phase RNA purification

Product Code. 15443435
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Quantity:
200 mL
Unit Size:
200mL
This item is not returnable. View return policy

Product Code. 15443435

Brand: Macherey-Nagel™ 740404.200

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This item is not returnable. View return policy

Description

Total RNA extraction from all kinds of sample.

NucleoZOL is designed for the isolation of total RNA (small and large RNA) in a single fraction or in separate fractions from a variety of sample materials.

  • No chloroform, no phase separation, easy procedure
  • High RNA yield and purity from any sample material
  • RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
  • Small and large RNA in one or in separated fractions
  • RNA ready for use in a variety of downstream applications: RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Sample material:

  • < 1 x 107 cultured cells, bacteria, and yeast
  • < 100 mg human/animal/plant tissue
  • < 0.4 mL (viral) fluids

Typical yield:

  • Total RNA
    • Liver: 6-8 μg/mg tissue
    • Kidney, spleen: 3-4 μg/mg tissue
    • Muscle, brain, lung: 0.5-1.5 μg/mg
    • Cultured cells: 4-10 μg/106 cells
  • Large RNA
    • Liver: 5-7 μg/mg tissue
    • Kidney, spleen: 3-4 μg/mg tissue
    • Muscle, brain, lung: 0.5-1.5 μg/mg
    • Cultured cells: 3-8 μg/106 cells

Specifications

CAS 593-84-0; 615-004-00-3
Color Blue
Content And Storage Room temperature (18 - 25 °C)
Isolation Technology One-phase Extraction
Sample Type Animal/Human/Plant Tissue, Bacteria, Cultured Cells, Viral Fluids, Yeast
Efficiency Typical RIN: > 9
Final Product Type Total RNA
Formulation Phenol, Guanidinium thiocyanate, Dye
For Use With (Application) RNA isolation from cells and tissue
Odor Aromatic
Purity A260/A280: 1.8 - 2.1
Quantity 200 mL
Sample Size <2 x 106 cultured cells, <2 x 106 bacterial cells, <2 x 106 yeast cells, <100 mg human /animal/plant tissue, <0.4 mL viral fluid
Test Time <1 h
Form Liquid
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Safety and Handling

compliance-icons compliance-icons compliance-icons compliance-icons
Product Identifier
  • nucleozol
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Skin corrosion/irritation Category 1 B
  • Germ cell mutagenicity Category 2
  • Specific target organ toxicity after repeated exposure Category 2
  • LONG-TERM AQUATIC HAZARD Chronic 3
 Hazard Statement
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H314-Causes severe skin burns and eye damage.
  • H331-Toxic if inhaled.
  • H341-Suspected of causing genetic defects.
  • H373-May cause damage to organs.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
 Supplemental information
  • MIXTURE LIST-Contains : phenol, guanidiniumthiocyanate
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