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Macherey-Nagel™ NucleoSpin™ RNA Plus, Mini kit for RNA purification with DNA removal column
Description
During the isolation of RNA, it is imperative to prevent the degradation of the RNA. In the NucleoSpin™ RNA Plus method, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases. The lysate is added to the gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After the addition of the Binding Solution to the flow-through, the RNA is bound to the RNA Plus Column. Two subsequent wash steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H2O.
- Includes NucleoSpin™ gDNA Removal Column: no time consuming rDNase digestion
- Convenient handling: lysate clearing and gDNA removal with one column in one step
- New efficient lysis buffer: no β-mercaptoethanol or TCEP necessary
- Downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
- Kit contents: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes, buffers
Specifications
Specifications
| Bacterial Content | <109 Cells |
| Content And Storage | Room temperature (18-25°C) |
| Buffer | Lysis Buffer |
| Format | Mini Spin Column |
| Isolation Technology | Silica Membrane System (Two Columns) |
| Elution Volume | Elution volume: 30–120μL |
| Sample Type | <1 x 107 Cultured Cells, <1 x 109 Bacterial Cells, <1 x 108 Yeast Cells, <30 mg Human/Animal Tissue |
| For Use With (Application) | RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms |
| Includes | NucleoSpin™ RNA Plus Columns with Collection Tubes, NucleoSpin™ gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers |
| No. of Reactions | 10 |
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Safety and Handling
- Warning
- Flammable liquid Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
- MIXTURE LIST-Contains : ethyleneglycol diacetate / guanidinium thiocyanate /guanidine hydrochloride, ethanol
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