Macherey-Nagel™ NucleoSpin™ Filters with Collection Tubes

In the NucleoSpin™ method, cells are lysed by incubation in a solution containing chaotropic ions to  inactivate RNases, creating appropriate binding conditions for adsorption of RNA to the silica membrane.
After lysis, thorough homogenisation and reduction of viscosity are achieved by filtration with NucleoSpin™ filter units. Contaminating DNA bound to the membrane is removed by a DNase I solution directly applied to the membrane. Optimal conditions for DNase are achieved by washing the membrane with a specific desalting buffer before treatment. Salts, metabolites and macromolecular cellular components are removed by simple washing with two different buffers. Total RNA is finally eluted with RNase-free water.

• Silica membrane technology
• Yield: 3μg to 70μg from 100μg plant material
• Sample material: 1mg to 100mg tissue
• Elution volume: 60μL
• Fragment size: 200b to 20kb
• Binding capacity: 200μg
• Preparation time: 30min/6 preps
• Format: mini spin column
• DNase I included
• NucleoSpin filter columns included for homogenisation of lysate and reduction of viscosity
• Parallel purification of DNA possible by using the NucleoSpin RNA/DNA buffer set NucleoSpin™ RNA Plant is designed for isolation of DNA-free high quality RNA from a wide variety of plant and fungal samples.