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  6. Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Product Code. 15533636
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No. of Samples:
24 x 96
4 x 96
Product Type:
Core Kit
Full Kit
Unit Size:
2304 reactions
384 reactions
3 product options available for selection
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Product Code. No. of Samples Product Type Unit Size
15533636 4 x 96 Full Kit 384 reactions
15553636 4 x 96 Core Kit 384 reactions
15543636 24 x 96 Core Kit 2304 reactions
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Product Code. 15533636 Supplier Macherey-Nagel™ Supplier No. 740491.4
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Descrizione

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
  • Ultra-fast procedure simplifies the work-flow
  • High-throughput — 96-well plates allow for easy automation and supports plasmid screening
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifica

Bacterial Endotoxins <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Format 96-well Plate
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Sample Type Bacterial Culture (E. coli, High-copy Plasmids)
Efficiency Binding capacity: 20 μg
Elution Volume Elution Volume: 100 - 200 μL
Final Product Type Transfection-grade plasmid DNA
For Use With (Equipment) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
For Use With (Application) Rapid purification of plasmids for transfection
Includes Filter Plates, Binding Plates, MN Wash Plates, Culture Plates, Elution Plates, Buffers, RNase A
No. of Samples 4 x 96
Product Type Full Kit
Purity A260/A280: 1.80 - 1.85
Sample Volume Up to 5 mL
Size < 15 kbp
Test Time 45 min/plate
Yield 5 - 20 μg
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Sicurezza e movimentazione

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Identificativo prodotto
  • NucleoSpin 96 Plasmid T.-grade (4x96)
 Avvertenza
  • Danger
 Categoria di pericolo
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Indicazioni di pericolo
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Consigli di prudenza
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Informazioni supplementari
  • MIXTURE LIST-Contain: sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse
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