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Macherey-Nagel™ NucleoSnap Plasmid Midi kit for plasmid DNA

Product Code. 15573636
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50 Preps
This item is not returnable. View return policy

Product Code. 15573636

Brand: Macherey-Nagel™ 740494.50

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This item is not returnable. View return policy

Description

Ultrafast plasmid Midi prep due to vacuum processing

NucleoSnap™ Plasmid Midi Columns

  • Produces ready-to-use, transfection-grade DNA
  • Isolates up to 250 μg plasmid DNA in only 35 minutes
  • No alcohol precipitation necessary
  • Easy procedure: DNA precipitation – filtration – washing – elution
    • High-salt / ethanolic washing steps to remove contaminants
    • Low-salt elution
  • Typical downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application such as cloning and sequencing

Specifications

Bacterial Endotoxins <50 EU/μg DNA
Color Clear
Format Snap-off Column
Isolation Technology Silica Membrane Technology
Sample Type E. coli Culture
Efficiency Binding capacity: 1.5 mg
Elution Volume Elution volume: 200 - 500 μL
Final Product Type Transfection-Grade Plasmid DNA
For Use With (Equipment) NucleoVac 24 Vacuum Manifold
For Use With (Application) Isolation of plasmid DNA
Includes NucleoSnap Plasmid Columns, NucleoSpin Plasmid Filter Columns, Buffers, RNase A
No. of Reactions 50 Preps
Purity A260/A280: 1.8 - 1.9
Sample Volume Up to 50 mL
Size ≤ 25 kbp
Test Time 35 min/6 preps
Yield 250 μg
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Safety and Handling

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Product Identifier
  • NucleoSnap Plasmid Midi
 Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : RNase; sodium hydroxide solution (diluted < 2 %), 2-propanol
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