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Macherey-Nagel™ NucleoBond™ Xtra BAC

Product Code. 12723401
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12723401 25 Preps
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Product Code. 12723401 Supplier Macherey-Nagel™ Supplier No. 740436.25

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Description

The second generation of anion exchanger designed for ultra fast purification of very large construct plasmid DNA (P1, BACs, PACs) ranging from 3 kbp up to 300 kbp.

NucleoBond™ Xtra BAC — For transfection-grade, large construct plasmid DNA

  • Optimized column design – one prep in approximately 75 min
  • Column filter included – parallel lysate clearing and loading onto the column
  • Optimal silica material – yields up to 150 μg
  • Transfection-grade plasmid DNA using proven anion-exchange technology
  • LyseControl for visualization of a completed lysis / neutralization
  • Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection

Specifications

Content And Storage Room temperature (18°C to 25°C)
Format Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
Sample Type E. coli Culture
Efficiency Binding capacity: 150 μg
Final Product Type Transfection-grade, large construct plasmid DNA
For Use With (Equipment) Smart Rack, Combi Rack or equivalent holder
For Use With (Application) Large construct DNA purification
Includes NucleoBond Xtra BAC Columns with inserted NucleoBond Xtra Column Filters, buffers, RNase A
No. of Reactions 25 Reactions
Purity A260/A280: 1.80 - 1.95
Sample Volume 250 to 750 mL
Size < 300 kbp
Test Time 75 min./2 to 4 Preps
Yield 10 to 150 μg
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contain: ethanol (diluted < 20 %) / sodium hydroxide solution (diluted < 2 %), dodecyl sulfate, sodium salt
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