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Invitrogen™ Rat IL-18 ELISA Kit

Product Code. 10561095
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Quantity:
96 Tests
Unit Size:
Each
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10561095

Brand: Invitrogen™ KRC2341

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554.80 EUR valid until 2025-12-31
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

ELISA

The Rat Interleukin 18 (Rt IL-18) ELISA quantitates Rt IL-18 in rat serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-18. Principle of the method The Rat IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P97636
Assay Range 15.6 to 1000 pg/mL
Assay Sensitivity <4 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Rat
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 29197
Gene Symbol Il18
Incubation Time 4 hr.
Interassay CV 4.9%
Intraassay CV 3.9
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene IL-18
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target IL-18
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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