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Invitrogen™ Nitric Oxide Assay Kit

Product Code. 10752345
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Quantity:
2 x 96 tests
Unit Size:
1 set
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10752345

Brand: Invitrogen™ EMSNO

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Beschreibung

Measure nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.

Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.

Additional Information and Online Ordering

Nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. The interaction of nitric oxide (NO) in a system is measured by the determination of both nitrate and nitrite concentrations in the sample. The relative levels of nitrite and nitrate can vary substantially, therefore the most accurate determination of total NO production requires measurement of both nitrate and nitrite.

  • Target: Nitric oxide (NO) and relative levels of nitrite (NO2-) and nitrate (NO3-)

  • Format: Colorimetric stain (azo dye) for clear 96-well plates

  • Measurement: Absorbance at 540nm (± 20nm)

  • Assay Range: 0 to 100μM

  • Sensitivity: 0.222μM nitrite; 0.625μM nitrate

  • Precision: Dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)

  • Sample Types: Urine, saliva, plasma, serum and other biological fluids

  • Total Assay Time: 15 minutes (nitrite assay); 45 minutes (nitrate assay)

  • Sample Size: 50μL

Includes:

Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

TRUSTED_SUSTAINABILITY

Spezifikation

Quantity 2 x 96 tests

Sicherheit und Handhabung

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Produktidentifikator
  • Nitric Oxide Assay Kit
 Signalwort
  • Warning
 Gefahrenkategorie
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Gefahrenhinweis
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Sicherheitshinweis
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Ergänzende Informationen
  • MIXTURE LIST-Contains : hydrogen chloride

For Research Use Only. Not for use in diagnostic procedures.

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