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Invitrogen™ MultiShot™ FlexPlate DH10B T1R Competent Cells
Description
Includes
Box 1:
- MultiShot™ FlexPlate DH10B™ T1R Chemically Competent E. coli
- One 96-well plate, 20 μl per well
Box 2:
- pUC19 DNA (10 pg/μl): 1 vial, 50 μl
- S.O.C. medium: 2 bottles, 10 ml each
MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be conveniently divided into 8-well segments for flexibility in transformation throughput.
All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.
Diverse DH10B T1R cloning capabilities
MultiShot FlexPlate DH10B T1R competent cells may be used in a variety of cloning applications as they are resistant to the effects of ligase and ligation buffers and provide transformation efficiencies of >1 x 108 cfu/μg with control plasmid DNA. The cells are ideal for cloning large plasmids and BACs and are suitable for cloning DNA that contains methylcytosine and methyladenine (i.e., genomic DNA).
MultiShot FlexPlate DH10B T1R Competent Cell features
• mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• endA1 for increased plasmid yield and quantity
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones
Increase transformation productivity with MultiShot FlexWell format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.
Key benefits of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible 96-well plate may be divided into 8-wellsstrips
• Competent cells come pre-aliquoted in 96-well plates and ready for transformation
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 lacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK rpsL nupG λ- tonA
Find the strain and format that you need
• We offer many E. coli strains and formats of chemically competent and electrocompetent cells to meet your specific transformation needs.
• Choose from our MultiShot formatted comp cells for high-throughput options.
Specifications
Specifications
| Product Type | Chemically Competent Cells |
| Contains F' Episome | No |
| Cloning Methylated DNA | Yes |
| Content And Storage | • MultiShot FlexPlate DH10B T1R Competent Cells (1 x 96-well plate at 20 μL/well) Store at –80°C. • pUC19 DNA (50 μL at 10 pg/μL) Store at –20°C. • S.O.C. Medium (2 x 10 mL) Store at 4°C or room temperature. |
| Antibiotic Resistance Bacterial | No |
| Blue/White Screening | Yes (lacZΔM15) |
| High-throughput Compatibility | High |
| Plasmid | May be used for plasmids >100 kb |
| Preparing Unmethylated DNA | No |
| Reduces Recombination | Yes (recA1) |
| Show More |
Frequently Asked Questions (FAQs)
There are situations where either because of time or process limitations, elimination of the heat shock and recovery time post-heat shock in the transformation protocol would be desirable. Testing was performed on many of our chemically competent cells in both the MultiShot StripWell and MultiShot FlexPlate format with a shortened transformation protocol. Use of a rapid transformation protocol (add DNA, no heat shock, no recovery period, plate on warm agar) yielded up to a log lower in transformation efficiency using the control DNA. This rapid transformation protocol can be performed with expectation of lower colony counts than our standard high efficiency transformation protocol and can only be performed with plasmids that use ampicillin as their antibiotic resistance. The rapid protocol is ideal when using a liquid handling system for high-throughput transformations with high cloning efficiency. Please see the product manual for full details on this rapid transformation protocol option.
The MultiShot FlexPlate is a PCR plate and can be placed in a thermal cycler or heat block set at 42 degrees C to transform the cells by the heat shock method. The duration of the heat shock performed in these devices has yielded similar transformation efficiencies as that seen with heat shock done in a water bath.
We offer the following E. coli strains in the MultiShot StripWell format: TOP10, DH5alpha T1R, Mach1 T1R, Stbl3, and BL21 Star (DE3) chemically competent cells.
We offer the following E. coli strains in the MultiShot FlexPlate format: TOP10, DH5alpha T1R, DH10B T1R, Mach1 T1R, OmniMAX2 T1R, and Stbl3 chemically competent cells.
The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.
The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.
The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.
For Research Use Only. Not for use in diagnostic procedures.
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