Invitrogen™ TNFR1 (Soluble) Mouse ELISA Kit

Description

The Mouse soluble TNFRI (TNFRSF1A) ELISA quantitates Ms TNFRI in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms sTNFRI. Principle of the method The Mouse TNF-RI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor necrosis factor receptor 1 (TNFR1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. This receptor plays an important role in mediating apoptosis. TNFR1 also plays an important role in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of the receptor is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.