missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 10371204

Thermo Scientific™ Pierce™ Mouse IL-12 (total) ELISA Kits

Product Code. 10371204
Click to view available options
Quantity:
2 Plate Kit
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10371204

Brand: Thermo Scientific™ Pierce™ EMIL12TOT2

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 47 to 3000pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 47 to 3000pg/mL
Assay Sensitivity <12pg/mL
Description Mouse IL-12 Total (p70, p40) ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Specificity No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
Sufficient For 2 x 96 microplate wells
Kit Contents TMB Substrate Solution, 2 x 12mL, Adhesive Plate Covers, 12 ea, Anti-Mouse IL-12 Total Precoated 96-well Strip Plate, 2 ea, Streptavidin-HRP Concentrate, 2 x 75 μL, Stop Solution, 2 x 13mL, Standard Diluent, 2 x 20mL, Lyophilized Recombinant Mouse IL-12 Standard, 4 vials, HRP Dilution Buffer, 2 x 13mL, 30X Wash Buffer, 2 x 50mL, Biotinylated Antibody Reagent, 2 x 12mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Dependent upon sample type (generally, intra- and inter-assay CV <10%)
Quantity 2 Plate Kit
Sample Volume 50 μL
Target Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Total Assay Time 3 hr.
Show More Show Less
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.