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Proteintech™ Mouse IL-12 p70 ELISA Kit

Product Code. 16851606 Shop All Proteintech Products
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Target Species:
Mouse
Unit Size:
96 tests
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Product Code. Target Species unitSize
16851606 Mouse 96 tests
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Product Code. 16851606 Supplier Proteintech™ Supplier No. KE1001496T

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Description

K10014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL12 p70.

  • The assay recognizes mouse IL12 p70. An antibody specific for mouse IL12 p70 has been pre-coated onto the microwells.
  • The mouse IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL12 p70 is added to detect the captured mouse IL12 p70 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
  • Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • Interleukin-12 (IL12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes.
  • IL12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL12a p35) and a 40 kDa subunit (IL12b p40).
  • IL12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells.
  • IL12 plays an important role in the activities of natural killer cells and T lymphocytes. IL12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
  • IL12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.

Specifica

Accession Number NP_032377 / NP_001290173
Assay Range 15.6 to 1000 pg/mL
Assay Sensitivity 2.2 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Mouse
Gene ID (Entrez) 16159
Host Species Mouse
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P43431 / P43432
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Sicurezza e movimentazione

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Identificativo prodotto
  • 96TEST Mouse IL-12 p70 ELISA Kit
 Avvertenza
  • Warning
 Categoria di pericolo
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Indicazioni di pericolo
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Consigli di prudenza
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Informazioni supplementari
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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