missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 16851616

Proteintech™ Mouse IL-1 alpha ELISA Kit

Product Code. 16851616 Shop All Proteintech Products
Change view
Click to view available options
Target Species:
Mouse
Unit Size:
96 tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Target Species unitSize
16851616 Mouse 96 tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 16851616 Supplier Proteintech™ Supplier No. KE1002496T

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

KE10024 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-alpha

  • Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body’s response to inflammation, microbial invasion, tissue injury and immunological response.
  • In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer’s disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation.
  • It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. . The assay recognizes mouse IL1-alpha.
  • An antibody specific for IL1-alpha has been pre-coated onto the microwells. The IL1-alpha protein in samples is captured by the coated antibody after incubation.
  • Following extensive washing, another antibody of biotinylated specific for IL1-alpha is added to detect the captured IL1-alpha protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Specifications

Accession Number NP_034684
Assay Range 7.8 to 500 pg/mL
Assay Sensitivity 2.7 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Mouse
Gene ID (Entrez) 16175
Host Species Mouse
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P01582
Show More Show Less

Safety and Handling

compliance-icons compliance-icons
Product Identifier
  • 96TEST Mouse IL-1 alpha ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.