Proteintech™ Mouse IFN-gamma ELISA Kit

Description

• The assay recognizes mouse IFN-γ. An antibody specific for IFN-γ has been pre-coated onto the microwells. The IFN-γ protein in samples is captured by the coated antibody after incubation.
• Following extensive washing, another antibody of biotinylated specific for IFN-γ is added to detect the captured IFN-γ protein.
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
• The IFNs were originally discovered as agents that interfere with viral replication. Initially, they were classified by the secreting cell type but are now classified into type I and type II according to receptor specificity and sequence homology.
• Interferon gamma (IFN-γ) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells.
• The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant Ifng expression is associated with a number of autoinflammatory and autoimmune diseases.
• It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.