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Proteintech™ Mouse Erythropoietin ELISA Kit

Product Code. 16821626 Shop All Proteintech Products
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Target Species:
Mouse
Unit Size:
96 tests
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Product Code. Target Species unitSize
16821626 Mouse 96 tests
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Product Code. 16821626 Supplier Proteintech™ Supplier No. KE1003196T

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Description

KE10031 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Erythropoietin ELISA kit is to be used to detect and quantify protein levels of endogenous Erythropoietin.

  • The assay recognizes mouse Erythropoietin. An antibody specific for Erythropoietin has been pre-coated onto the microwells.
  • The Erythropoietin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Erythropoietin is added to detect the captured mouse Erythropoietin protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
  • Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin.
  • The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC.
  • EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte.
  • However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue.
  • A complete lack of erythropoietin causes embryonic lethal anemia in mice. The conditional inactivation of erythropoietin in adult mice results in a chronic, normocytic and normochromic anemia. 

Specifications

Accession Number BC119265
Assay Range 78.1 to 5000 pg/mL
Assay Sensitivity 12 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Mouse
Gene ID (Entrez) 13856
Host Species Mouse
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P07321
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Safety and Handling

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Product Identifier
  • 96TEST Mouse IL-4 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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