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Invitrogen™ Vybrant™ Phagocytosis Assay Kit
Observe and quantitate process of phagocytosis in human polynuclear cells and mouse macrophages by following internalization of a foreign particle
Brand: Invitrogen™ V6694
Additional Details : Weight : 0.05000kg
Description
- Foreign particle is killed, E. coli (K-12 strain) cells that have been labeled with fluorescent dye fluorescein
- Addition to lyophilized E. coli BioParticles™ component, kit contains a trypan blue solution (to quench fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader
- Methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines
- Particles are fluorescein-labeled E. coli (K-12 strain)
- Kit supplies sufficient reagents for approx. 250 tests using 96-well plates
- Fluorescence is measured using a microplate reader (Ex/Em approx. 480/520nm)
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection
Order Info
Shipping Condition: Room Temperature
Specifications
| Store in freezer (-5 to -30°C) and protect from light. | |
| Microplate Reader, Microplate Reader | |
| Classic Dyes | |
| Vybrant™ | |
| E. coli |
| Fluorescent | |
| Phagocytosis Assay Kit | |
| 96-well plate | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.