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Invitrogen™ CyQUANT™ Cytotoxicity Assay Kit (G6PD Release Assay)

Product Code. 10666523
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1000 Assays
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10666523 1000 Assays Each
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For Research Use Only. All usage must comply with product instructions.
Product Code. 10666523 Supplier Invitrogen™ Supplier No. V23111

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.

CyQUANT cytotoxicity assays provide a simple and reliable method to assess chemical or cell-mediated cellular toxicity. The CyQUANT LDH Cytotoxicity Assays and the CyQUANT Cytotoxicity Assay (G6PD Release Assay) use colorimetric- or fluorescence-based detection to measure the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays are optimized for use on microplate readers and can be used for continuous monitoring of cell health over time since cell lysis is not required, making them versatile tools for high-throughput screening and drug discovery applications.

With the CyQuant G6PD Release Assay (V23111), damaged and dying cells release glucose 6-phosphate dehydrogenase (G6PD) into the surrounding medium. G6PD is detected through a two-step enzymatic process via oxidation of glucose 6-phosphate by G6PD to generate NADPH, which leads to the reduction of resazurin by diaphorase to red-fluorescent resorufin. This assay results in lower background signals than are typically observed in LDH assays, offering increased sensitivity. It can detect as few as 500 cells and is more sensitive than LDH release assays.

Features of the CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay)

  • High sensitivity—detects as few as 500 cells, offering greater sensitivity than LDH release assays
  • Specific detection—measures glucose 6-phosphate dehydrogenase (G6PD) release from damaged and dying cells
  • Low background signals—produces lower background fluorescence compared to LDH release assays
  • Accurate and reliable—the fluorescence signal (ex/em 563/587 nm) is proportional to the number of dead cells, ensuring accurate cytotoxicity measurement

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Permeability Cell-impermeant
Color Red
Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cytotoxicity Assay Kit (G6PD Release Assay)
For Use With (Equipment) Microplate Reader
Product Type G6PD Release Cytotoxicity Assay
Dye Type Other Label(s) or Dye(s), Resorufin
Emission 587 nm
Excitation Wavelength Range 563 nm
Format 96-well plate
Product Line CyQUANT
Quantity 1000 Assays
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.

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