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Invitrogen™ Rhod-5N, Tripotassium Salt, cell impermeant

Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipet, microinjection or pinocytotic cell-loading reagent

Brand:  Invitrogen™ R14207

299.34 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.00525kg

Product Code. 11563677

  • 449.00 € / 500µg
Estimated Shipment: 18-04-2024
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For Research Use Only. All usage must comply with product instructions.


Description

Description

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.

  • Fluorescence signal from these cells is generally measured using fluorescence microscopy
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in absence of Mg2+, in buffer: approximately 320μM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of these ions can be controlled using heavy metal-selective chelator TPEN

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Store in freezer -5°C to -30°C and protect from light.
Fluorescence Microscope
Fluorescent Dye-Based
Fluorescent
Calcium Indicator
Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.