Invitrogen™ RediPlate™ 96 EnzChek™ Serine/Threonine Phosphatase Assay Kit

The RediPlate 96 EnzChek Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.

Drug Discovery and Development, Enzyme and Protein Activity Assays, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation