Invitrogen™ Oregon Green™ 488 BAPTA-1 dextran, Potassium Salt, 10,000 MW, Anionic

Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca²⁺. They have uses in many calcium signaling investigations, including measuring intracellular Ca²⁺, following Ca²⁺ influx and release, and multiphoton excitation imaging of Ca²⁺ in living tissues.

• Cells may be physically loaded with cell-impermeant salt forms of these dextran-conjugated indicators using patch pipet or microinjection
• Fluorescence signal from these cells is measured using fluorescence microscopy
• Label (Ex/Em): Oregon Green 488 BAPTA-1 (494/523nm)
• Fluorescence intensity increase upon binding Ca²⁺: approximately 14 fold
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
• Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of problem

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling