Invitrogen™ Amplex™ Acetylcholine/Acetlycholinesterase Assay Kit

The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer. AChE activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. AChE converts the acetylcholine substrate to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

• Little interference from autofluorescence in most biological samples
• Detect AChE activity levels as low as 0.002U/mL in one hour
• Detect acetylcholine levels as low as 0.3μM using excess AChE
• Achieve detection ranges of 0.3μM to 100μM acetylcholine
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
• Custom assay design and packaging are also available

Drug Discovery and Development, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation