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Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Product Code. 10665605
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Product Type:
T3 In Vitro Transcription kit
No. of Reactions:
25 Reactions
Unit Size:
25 reactions
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Product Code. Product Type No. of Reactions unitSize
10665605 T3 In Vitro Transcription kit 25 Reactions 25 reactions
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For Research Use Only. All usage must comply with product instructions.
Product Code. 10665605 Supplier Invitrogen™ Supplier No. AM1348
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For Research Use Only. All usage must comply with product instructions.

Description

Obtain 10–50 times yield of conventional in vitro transcription reactions.

mMESSAGE mMACHINE high yield capped RNA transcription kits use patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

  • High yields of capped RNA—up to 35 μg of RNA per reaction
  • Extra convenience—cap analog-nucleotide mix provided
  • Simplified reaction format
  • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection
  • 10–50 times yield obtained with conventional in vitro transcription reactions
  • Ratio of cap analog to GTP has been optimized to allow compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%)
  • Contains a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
  • Optimized for use with polymerases included in kit
  • Contains 57 U of cap analog
TRUSTED_SUSTAINABILITY

Spécification

Content And Storage T3 Enzyme Mix, 10X Reaction Buffer 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions 25 Reactions
Product Line Ambion, mMESSAGE mMACHINE
Product Type T3 In Vitro Transcription kit
Quantity 25 Reactions
Promoter T3
Shipping Condition Dry Ice
Yield 20-30 μg
Starting Material PCR Products, Linearized DNA plasmid
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For Research Use Only. Not for use in diagnostic procedures.

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