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Description
This MicroSEQ™ ID Purification Combo Kit includes reagents for post-PCR and post-sequencing steps in the MicroSEQ™ ID microbial identification workflow. It contains 2 tubes of ExoSAP-IT™ Reagent for PCR clean-up and 36 MicroSEQ™ ID Sequencing Clean-up Cartridges.
ExoSAP-IT™ Reagent: this reagent is used for quick post-PCR cleanup for downstream applications such as sequencing. It allows for removal of unconsumed dNTPs and primers remaining in the PCR mix following amplification.
ExoSAP-IT™ Reagent can be used downstream of the following PCR amplification kits:
- MicroSEQ™ 500 16S rDNA PCR Kit
- MicroSEQ™ Full Gene 16S rDNA PCR Kit
- MicroSEQ™ D2 rDNA Fungal PCR Kit
MicroSEQ™ ID Sequencing Clean-up Cartridges: these cartridges are used for post-sequencing clean-up in the MicroSEQ™ ID workflow. The gel filtration matrix used in the cartridges provide a convenient and simple way to remove dye terminators, dNTPs, and salts, as well as residual primers from cycle sequencing reactions.
The MicroSEQ™ ID Sequencing Cleanup Cartridges can be used downstream of the following sequencing kits:
- MicroSEQ™ 500 16S rDNA Sequencing Kit
- MicroSEQ™ Full Gene 16S rDNA Sequencing Kit
- MicroSEQ™ D2 rDNA Fungal Sequencing Kit
Specifications
Specifications
| Format | Spin Column |
| Content And Storage | The kit contains an Exo SAP-IT PCR Purification kit and DTR Ultra Cartridge. |
| Final Product Type | DNA |
| For Use With (Application) | Sequencing |
| Isolation Technology | Enzymatic, Spin Column |
| Product Line | MicroSEQ |
| Purification Target | Sequencing Reactions |
| Sample Type | Double-Stranded DNA (dsDNA) |
| System Type | MicroSEQ™ ID |
| Product Type | ID Purification Combo Kit |
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For Research Use Only. Not for use in diagnostic procedures.
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