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Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red)
A two-color fluorescent cell viability assay that simultaneously identifies live and dead cells. Live cells are labeled with calcein dye and emit a green fluorescence, while dead cells are labeled with SYTOX Deep Red dye and emit a red fluorescence.
Brand: Invitrogen™ L32250
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Description
LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red)
- A two-color fluorescent cell viability assay that simultaneously identifies live and dead cells
- Live cells are labeled with calcein dye and emit a green fluorescence
- Dead cells are labeled with SYTOX Deep Red dye and emit a red fluorescence
- Dyes can be multiplexed with other orange/red fluorophores with compatible filter sets
- Kit is suitable for use with fluorescence microscopes or high content analysis (HCA) fluorescence multiwall plate scanners
- Easily adaptable for use other fluorescence detection systems
- Assay principles are general and applicable to most eukaryotic cell types, including adherent and non-adherent cells
Specifications
| Green, Deep Red | |
| LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) | |
| Viability Assay | |
| M25 | |
| 495, 660 nm | |
| Calcein, AM (live cell indicator), SYTOX™ Deep Red Nucleic Acid Stain (dead cell indicator) | |
| 2 x 40 μL Vials | |
| Viability |
| • 1 vial of Calcein AM (live cell indicator), 40 μL, provided as a 2000X (4 mM) solution in DMSO • 1 vial of SYTOX Deep Red Nucleic Acid Stain (dead cell indicator), 40 μL, provided as a 2000X (500 μM) solution in DMSO Store at ≤-20°C, upright in dry place. Protect from light. |
|
| Fluorescence | |
| Fluorescence Microscope, High Content Analysis Instrument, Other Fluorescence Detection Systems | |
| 515, 682 nm | |
| Kit | |
| LIVE/DEAD | |
| Room Temperature |