missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. Nucleic Acid Labeling and Detection
  5. 10188593

Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit

Product Code. 10188593 Shop All Thermo Scientific Products
Change view
Click to view available options
:
100 reactions
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code.
10188593 100 reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Product Code. 10188593 Supplier Thermo Scientific™ Supplier No. 20148

Please to purchase this item. Need a web account? Register with us today!

612.03 EUR valid until 2026-03-13
BEST PRICE on Promo! Use promo code "27968" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Description

Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system.

Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions.

The kit includes reagents for setting up and customizing protein:DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection.The principle for LightShift™ EMSA Detection is similar to a Western blot. Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel. The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV-crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate. The protocol from labeling to results can be accomplished in a single day. All you need to perform the assay are purified DNA target that has been end-labeled with biotin, the protein extract you wish to test, nylon membrane and basic electrophoresis equipment. DNA targets may be synthesized with 5' or 3' biotin labels or they may be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Part No. 89818). Nuclear, cytosolic or whole cell protein extracts may be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Part No. 78833).

Highlights:

  • Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity
  • Excellent for detecting low-abundance proteins in nuclear extracts
  • Sensitivity that surpasses radioactive and digoxigenin methods
  • Compatible with previously established binding conditions for popular DNA:protein interactions
TRUSTED_SUSTAINABILITY

Specifications

Formulation

Multi-component kit with reagents for DNA-protein binding reactions and blot detection
For Use With (Application) Electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
Includes LightShift Optimization and Control Kit (20148X), 1 kit; Chemiluminescent Nucleic Acid Detection Module (89880), 1 kit
No. of Reactions 100 binding reactions and detection on 800cm2 of membrane (10 mini-gel blots)
Product Type LightShift Chemiluminescent EMSA Kit
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.