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Thermo Scientific™ Klenow Fragment (10 U/μL)
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.
86.00 € - 345.00 €
Specifications
| Concentration | 10 U/μL |
|---|---|
| Polymerase | DNA Polymerase I |
| Product Type | Klenow Fragment |
| Product Code | Brand | Concentration | Quantity | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Concentration | Quantity | Price | Quantity & Availability | ||||
|
10130800
|
Thermo Scientific™
EP0051 |
10 U/μL | 300 U |
86.00 €
300 units |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
10795071
|
Thermo Scientific™
EP0052 |
10 U/μL | 1500 U |
345.00 €
1500 units |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
Description
Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.
Highlights
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
- Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers
Applications
- DNA blunting by fill-in 3'-overhangs
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA
- DNA sequencing by the Sanger method
- Site-specific mutagenesis of DNA with synthetic oligonucleotides
- Second strand synthesis of cDNA
Specifications
| 10 U/μL | |
| DNA Polymerase I | |
| Klenow Fragment |
For Research Use Only. Not for use in diagnostic procedures.