Invitrogen™ T4 DNA Polymerase

• Applications: Labeling double-stranded linear DNA by replacement synthesis, Oligonucleotide-directed, site-specific mutagenesis, 3' end-labeling of double-stranded DNA, Polishing both 5' or 3 overhangs to make blunt ends
• Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid
• Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested
• Unit Definition: One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template primer
• Unit Reaction Conditions: 50mM glycine-NaOH (pH 8.8), 16.6mM (NH₄)₂SO₄, 6mM MgCl₂, 6.5μM EDTA, 10mM 2-mercaptoethanol, 0.165mg/mL BSA, 1.6mg/mL DNase I-nicked salmon testes DNA, 0.33mM dCTP, 0.33mM dATP, 0.33mM dGTP, 0.33mM dTTP, 76nM [^{3H]dTTP, and enzyme in 0.1mL for 30 min. at 37°C}
• A T4 DNA Polymerase Technical Bulletin is available

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Restriction Enzyme Cloning