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Invitrogen™ SuperScript™ Plus Direct cDNA Labeling System with Alexa Fluor™-aha-dUTPs

Product Code. 10226952
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Product Code. 10226952 Supplier Invitrogen™ Supplier No. L101506

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Description

Includes

All SuperScript™ Direct cDNA Labeling Systems include SuperScript™ III Reverse Transcriptase, anchored Oligo(dT), random primers, control RNA, 5X first-strand buffer, 0.1M DTT, 10mM dNTP mix, low-elution purification columns, loading buffer, wash buffer, amber collection tubes, and DEPC-treated water. The SuperScript™ Plus Direct cDNA Labeling Systems additionally include Alexa Fluor™ 555- and 647-aha-dUTP. The SuperScript™ Direct cDNA Labeling Modules contain all the same components with the exception of the purification components.

A microarray labeling kit that combines the superior performance of SuperScript III Reverse Transcriptase (RT) with the simplicity of direct labeling, plus Alexa Fluor™-aha-dUTPs

In place of an enzyme spike-in, the streamlined protocol incorporates balanced sets of novel, array-optimized, nucleotides conjugated to Alexa Fluor 555 and 647 dyes. This results in more reproducible array data with higher correlation coefficients than using standalone reagents.

In the SuperScript Plus cDNA Labeling method, anchored oligo(dT) anneals to the mRNA template. SuperScript III RT extends from the priming site, incorporating dUTP labeled with Alexa Fluor dye directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using low-elution volume purification columns before hybridization to the microarray.

  • Optimized, all-inclusive direct labeling with balanced aha-Alexa Fluor nucleotides, enabling higher numbers of array positives
  • Improved correlation coefficients for more accurate data
  • Low elution purification columns included for ease of use

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis

TRUSTED_SUSTAINABILITY

Specifications

Product Type cDNA Labeling System
Detection Method Fluorescence
Content And Storage All SuperScript™ Direct cDNA Labeling Systems/Modules include SuperScript™ III Reverse Transcriptase, anchored Oligo(dT), random primers, control RNA, 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mix, low-elution purification columns, loading buffer, wash buffer, amber collection tubes, and DEPC-treated water. The SuperScript™ Plus Direct cDNA Labeling System/Module additionally include Alexa Fluor™ 555- and 647-aha-dUTP. The 'System' also includes purification components. See the 'Module' (L101604) if purification components are not needed.

Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored.
Final Product Type cDNA (Labeled)
Label or Dye Alexa Fluor™ 555, Alexa Fluor™ 647
Includes Label or Dye Yes
Labeling Target cDNA
Labeling Method Direct Labeling
Sample Type RNA
Product Line Alexa Fluor, SuperScript
Quantity 30 Reactions
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For Research Use Only. Not for use in diagnostic procedures.

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