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Invitrogen™ SuperScript™ Direct cDNA Labeling Module, no purification
Description
Includes
The SuperScript™ Direct cDNA Labeling Module includes SuperScript™ III Reverse Transcriptase, Anchored Oligo(dT), Random Primers, control RNA, 5X First-Strand Buffer, 0.1M DTT, 10mM dNTP Mix, and DEPC-treated water. The SuperScript™ Direct cDNA Labeling System includes all components listed for the SuperScript™ cDNA Labeling Module plus purification columns, loading buffer, wash buffer, and amber collection tubes.
The SuperScript Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module. Modules do not include all the components. The SuperScript Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript III Reverse Transcriptase (RT) with the simplicity of direct labeling to yield highly fluorescent labeled cDNA that accurately represents your initial mRNA sample. In this method, anchored oligo(dT) anneals to the mRNA template. SuperScript III RT extends from the priming site, incorporating fluorescent dNTPs directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using purification columns before hybridization to a microarray.
- Provides the flexibility to use fluorescent nucleotides
- High cDNA yields without an additional enzyme “spike”
- Strong signal intensities
- A fast and simple protocol that minimizes “hands on” time and provides superior reproducibility
DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis
Spécification
Spécification
| Product Type | cDNA Labeling Module |
| Detection Method | Fluorescence |
| Content And Storage | The SuperScript™ Direct cDNA Labeling Module includes SuperScript™ III Reverse Transcriptase, Anchored Oligo(dT), Random Primers, control RNA, 5X First-Strand Buffer, 0.1 M DTT, 10 mM dNTP Mix, and DEPC-treated water. The SuperScript™ Direct cDNA Labeling System includes all components listed for the SuperScript™ cDNA Labeling Module plus purification columns, loading buffer, wash buffer, and amber collection tubes. Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored. |
| Final Product Type | cDNA (Labeled) |
| Label or Dye | Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5 |
| Includes Label or Dye | No |
| Labeling Target | cDNA |
| Labeling Method | Direct Labeling |
| Sample Type | RNA |
| Product Line | SuperScript |
| Afficher plus |
For Research Use Only. Not for use in diagnostic procedures.
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