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Invitrogen™ Proteinase K Solution

Code produit 15314118
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Quantity:
5 mL
Conditionnement:
5mL
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Code produit Quantity Conditionnement
15314118 5 mL 5mL
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For Research Use Only. All usage must comply with product instructions.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price
Code produit 15314118 Fournisseur Invitrogen™ Code fournisseur 4333793
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163.33 EUR valid until 2026-09-25
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Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.

For Research Use Only. All usage must comply with product instructions.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price

Description

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

  • Over a wide pH range (optimal activity between 6.5 and 9.5)
  • Under denaturing conditions (e.g., the presence of SDS or urea)
  • In the presence of metal chelating agents (e.g. EDTA)
  • At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and Quality Testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit Definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Informations sur la commande

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Spécification

Purity DNase-Free, RNase-Free
For Use With (Application) Chromatin Biology/RNA Extraction/DNA Extraction
Content And Storage Store in refrigerator (2°C to 8°C).
Shipping Condition Wet Ice
Quantity 5 mL
Product Type Proteinase K
Form Liquid

Frequently Asked Questions (FAQs)

How do you recommend storing proteinase K?

We recommend storing it at 4°C.
How do I isolate DNA from single bacterial colonies before PCR?

Scrape a single colony from the surface of an agar plate and transfer to 12 µL of SCL solution [10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 50 µg/mL proteinase K] and incubate for 15 min at 55 degrees C. Inactivate proteinase K for 15 min at 80 degrees C and add 20 µL of deionized water. Centrifuge at 12,000 x g for 3 min. Transfer the supernatant to a new tube.

For Research Use Only. Not for use in diagnostic procedures.

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