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Invitrogen™ pPICZ A, B, & C Pichia Vectors

Product Code. 17124629
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Quantity:
20 μg
Unit Size:
20µg
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17124629 20 μg 20µg
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For Research Use Only. All usage must comply with product instructions.
Product Code. 17124629 Supplier Invitrogen™ Supplier No. V19020

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

The EasySelect PichiaExpression Kit includes 20μg each of pPICZ A, B, and C and pPICZ A, B, and C, Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression, 250mg Zeocin , media (YP and YNB), EasyComp PichiaTransformation Kit, 2μg each 5' AOX1, 3' AOX1,and α-factor sequencing primers. A lacZ positive expression control is provided with pPICZ.

Designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein

  • These vectors contain Zeocin™ resistance gene for direct selection of multi-copy integrant strains
  • By selecting with increasing amounts of Zeocin, strains with multiple copies of the gene of interest integrated into genome are obtained
  • Increasing number of copies of gene of interest in recombinant Pichiastrain can result in higher expression levels
  • Inducible AOX1promoter for high-level expression in Pichia pastoris c-mycepitope tag for convenient detection with Anti-mycAntibody
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • The pPICZ vector also features secretion signal for efficient transport of proteins to medium

Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression

TRUSTED_SUSTAINABILITY

Spécification

Promoter AOX1
Product Type Pichia Expression Vector
Content And Storage 20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.

Store vectors at -5 to -30°C.
Antibiotic Resistance Bacterial Zeocin™ (ZeoR)
Protein Tag His Tag (6x), c-Myc Epitope Tag
Cloning Method Restriction Enzyme/MCS
Quantity 20 μg
Vector pPIC

For Research Use Only. Not for use in diagnostic procedures.

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