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  5. Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit 

Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Product Code. 10605583
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Quantity:
20 Reactions
40 Reactions
Unit Size:
Each
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10605583

Brand: Invitrogen™ K490001

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861.90 EUR valid until 2025-12-19
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

  • Proofreading enzyme is used: fewer errors in cloned genes
  • Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
  • CMV promoter: high expression levels
  • C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
  • 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type TOPO Expression Kit
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage Box 1 (Store at -20°C):
• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 20 μl T7 Sequencing Primer (0.1 μg/μl)
• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
• 10 μl Control PCR Template (0.1 μg/μl)
• 10 μl Control PCR Primers (0.1 μg/μl each)
• 10 μl Expression Plasmid (0.5 μg/μl)

Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 μl TOP10 Cells
• 50 μl pUC19 Control DNA
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Directional TOPO
Quantity 20 Reactions
Vector Directional TOPO Vectors, pcDNA
Product Line TOPO™, pcDNA™
For Use With (Application) Constitutive Expression
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For Research Use Only. Not for use in diagnostic procedures.

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