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Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells

MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.

Brand:  Invitrogen™ 10268019

299.34 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.02000kg

Product Code. 10799374

  • 449.00 € / Each
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For Research Use Only. All usage must comply with product instructions.


Includes: MAX Efficiency Stbl2 Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, SOC Medium: 1 bottle, 6mL

Description

Description

MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. The Stbl2 strain was used for the stable propagation of a plasmid containing approximately 100 direct repeats of 32 bp as well as full-length infectious simian immunodeficiency virus (SIV) clone can be maintained. This strain has also been demonstrated to stably propagate plant virus sequences such as potato virus Y (PVY).

MAX Efficiency Stbl2 Competent Cells are a derivative of the JM109/J5 strain. Competent cells are prepared by a patented modification of the procedure of Hanahan. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow for the cloning of methylated genomic sequences.

MAX Efficiency Stbl2 Competent Cells offer:
• >1 x 109 transformants/μg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA that allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeats and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Genotype
F- mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 lon gyrA96 thi supE44 relA1 λ- Δ(lac-proAB)

Note: For optimal performance, expression in S.O.C. Medium and incubation on antibiotic plates at 30°C is recommended.

Find the strain and format that fits your needs
• A variety of Stbl strains are available with different genetic background or suitable for electroporation.
• The Stbl3 strain is available in MultiShot format for high throughput applications.

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Chemically Competent Cells
Yes (endA1)
High Efficiency (>1 x 109 cfu/μg)
No
No
May be used for plasmids >20 kb
Yes (recA1)
No
Tube
5 x 200 μL
No
Yes (mcrB, mrr)
• MAX Efficiency Stbl2 Competent Cells (5 x 200 μL); store at –80°C
• pUC19 DNA (100 μL at 0.01 μg/mL); store at –20°C
• S.O.C. Medium (6 mL); store at 4°C or room temperature

Yes (recA1)
Low
No
Dry Ice
E. coli (K12)
MAX Efficiency™
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For Research Use Only. Not for use in diagnostic procedures.