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Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
430.00 € - 1722.00 €
Specifications
Format | Stand-alone Enzyme |
---|---|
Reaction Speed | 50 min. |
Technique | Reverse Transcription |
Optimal Reaction Temperature | 37°C |
Reverse Transcriptase | M-MLV |
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
10338842
|
Invitrogen™
28025013 |
200 Reactions |
430.00 €
Each |
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10247352
|
Invitrogen™
28025021 |
1,000 Reactions |
1722.00 €
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT)25 as template-primer.
Features of M-MLV Reverse Transcriptase include:
• Excellent thermostability with optimal activity at 37°C
• Can be used to synthesize first-strand cDNA up to 7 kb
• Multiple applications including synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
Source
Purified from E. coli expressing the pol gene of M-MLV on a plasmid
Performance and quality testing
SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 μL for 10 min at 37°C
Order Info
Shipping Condition: Wet ice
Specifications
Stand-alone Enzyme | |
Reverse Transcription | |
M-MLV | |
Wet Ice | |
First-Strand cDNA | |
Reverse Transcription | |
RNA |
50 min. | |
37°C | |
Yes | |
200 U/μL | |
Separate components | |
Up to 7 kb |
For Research Use Only. Not for use in diagnostic procedures.
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