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Invitrogen™ Expressway™ Mini Cell-Free Expression System

Product Code. 10666233
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10666233 1 Kit Each
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.
Product Code. 10666233 Supplier Invitrogen™ Supplier No. K990100

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

Designed to perform 20 x 50 μL reactions or 1 x 1mL reaction. This system includes the IVPS E.coliExtract, IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, RNase A, T7 Enzyme Mix, 2mL reaction tubes, and a positive expression control vector.

Uses efficient coupled transcription and translation reaction to produce active recombinant protein

  • Eliminates time-consuming steps of cell-based protein production including transformation, cell culture, and expression optimization
  • In two hours, can produce protein suitable for array of downstream applications and functional studies
  • Offers flexibility in experimental design
  • Synthesize proteins from both circular plasmid and linear templates (e.g. PCR products)
  • Perform high-throughput synthesis of proteins
  • Express genes toxic to in vivosystems
  • Provides all components need for optimal cell-free protein production
  • As DNA template is transcribed, 5« end of mRNA is bound by ribosomes and undergoes translation as 3« end of template is still being transcribed
  • Proprietary feed buffer formulation is provided with system for increased protein yields

Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Promoter T7
Product Type Cell-Free Expression System
Expressed Protein Type Water Soluble Protein
Expression System Cell-Free
Expression Mechanism Cell-Free Expression
Quantity 1 Kit
Product Line Expressway

Safety and Handling

compliance-icons
Product Identifier
  • Expressway Mini Cell-Free Expression System
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
 Supplemental information
  • MIXTURE LIST-Contain: Tris (hydroxymethyl)aminomethane

For Research Use Only. Not for use in diagnostic procedures.

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