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Invitrogen™ Dynabeads™ FlowComp™ Mouse CD4+CD25+ Treg Cells Kit

Product Code. 10721954
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Product Code. 10721954 Supplier Invitrogen™ Supplier No. 11463D

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Description

Includes

20mL Mouse Depletion Dynabeads, 2mL Antibody Mix, 1mL FlowComp; Dynabeads (mTreg), 0.3mL FlowComp; Mouse CD25 Antibody and 6mL FlowComp; Release Buffer

This gentle and easy-to-use kit allows you to isolate highly pure and functional mouse Treg cells from mouse lymph nodes or spleen samples.

This gentle and easy-to-use kit allows you to isolate highly pure and functional mouse Treg cells from mouse lymph nodes or spleen samples. Your cells are not exposed to the stress of being passed through a column. The isolated CD4+CD25+Foxp3+ Treg cells are bead-free and flow-compatible. The cells can be readily expanded and retain their functional suppressive capacity. You can expect a purity level of >90% Treg cells. Over 88% of the isolated CD25+ cells express Foxp3. You will save time and money, and get increased confidence in your results.

Methodology
Negative isolation of CD4+ T cells, followed by positive isolation of CD25+ regulatory T cells. First, an antibody mix is added to the sample and the Ab will bind the non-CD4+ T cells. Mouse Depletion Dynabeads™ are then added and will bind to the Ab-cell complex during a short incubation. Bead-bound cells are separated on a magnet and discarded. The remaining untouched mouse CD4+ T-cells are then labeled with FlowComp™ CD25 Antibody and positively isolated using FlowComp™ Dynabeads™. A short incubation with FlowComp™ Release Buffer then releases the isolated mouse regulatory T-cells from the Dynabeads™. The isolation method is easy to use and to scale up.

Advantages
High purity of CD4+CD25+Foxp3+ Treg cells.
• No risk of your results being affected by the isolation method
• Gentle tube-based method—no columns needed
• High purity, recovery, and viability
• High CD25+Foxp3+ expression.
• Bead-free cells—compatible with flow cytometry analysis
• Treg cells are readily expanded and retain their functional suppressive capacity.

Starting sample
Spleen or lymp node cells

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Type T Cells (Regulatory)
Content And Storage The Kit Contains:
• 20 mL Mouse Depletion Dynabeads™
• 2 mL Antibody Mix
• 1 mL FlowComp™ Dynabeads™ (mTreg)
• 0.3 mL FlowComp™ Mouse CD25 Antibody
• 6 mL FlowComp™ Release Buffer.

Store at: 2°C to 8°C.
Product Type Superparamagnetic Beads
Final Product Type Cells
Isolation Technology Negative & then Positive Isolation
No. of Cells Processes ∼1 x 109 cells total
No. of Tests 10 Isolations
Output Viability >95%
Product Line DYNAL, Dynabeads, FlowComp
Quantity 1 Kit
Reactivity Mouse
Sample Type Lymph Node, Spleen
Shipping Condition Room Temperature
Starting Material Cell No. 1 x 108 cells per isolation
Target Species Mouse
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For Research Use Only. Not for use in diagnostic procedures.

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